Head over to and sign up for an account. Spec2Vec, which we've written about in detail, is often a better measure than Cosine distance. But there are many other ways to do this. Cosine distance is one way to measure how similar to one another spectra are. In reality, there are many different ways for things to be similar to – or different from – each other. "Similarity" is often thought of as a fixed metric. Using Omigami to compute Spec2Vec similarity scores But interestingly, most of their cosine similarity scores are low. These other spectra are part of the Stenothricin cluster. Stenothricin C M+H has a 0.02 cosine match with Stenothricin I M+H (CCMSLIB00000075077) Stenothricin C M+H has a 0.04 cosine match with Stenothricin H M+H (CCMSLIB00000075076) Stenothricin C M+H has a 0.05 cosine match with Stenothricin E M+H (CCMSLIB00000075073) Stenothricin C M+H has a 0.04 cosine match with Stenothricin B M+H (CCMSLIB00000075072) Stenothricin C M+H has a 0.1 cosine match with Stenothricin D M+H (CCMSLIB00000075071) Stenothricin C M+H has a 0.03 cosine match with Stenothricin A M+H (CCMSLIB00000075070) If we happen to know the spectrumids of some other spectra in the Stenothricin cluster, we can find the similarity to each as follows: Because we used an example from the dataset as a query spectrum, we get one perfect match (with itself) and then some other close matches with Hydroxysprengerinin C and Stenothricin G. We print out the similarity between our “query” spectrum (Stenothricin C) and each of the top ten matches. In the for loop, we use to reverse the list as the more similar spectra are towards the end. Stenothricin C M+H has a 0.42 cosine match with 14-hydroxysprengerinin C + (CCMSLIB00006456701) Stenothricin C M+H has a 0.43 cosine match with 14-hydroxysprengerinin C + (CCMSLIB00006456917) Stenothricin C M+H has a 0.44 cosine match with 14-hydroxysprengerinin C + (CCMSLIB00006455914) Stenothricin C M+H has a 0.46 cosine match with 14-hydroxysprengerinin C + (CCMSLIB00006456712) Stenothricin C M+H has a 0.47 cosine match with 20(S)-Ginsenoside F2 + (CCMSLIB00006580075) Stenothricin C M+H has a 0.47 cosine match with 14-hydroxysprengerinin C + (CCMSLIB00006456985) Stenothricin C M+H has a 0.54 cosine match with 14-hydroxysprengerinin C + (CCMSLIB00006456977) Stenothricin C M+H has a 0.64 cosine match with Stenothricin G M+H (CCMSLIB00000075069) Stenothricin C M+H has a 0.76 cosine match with 14-hydroxysprengerinin C + (CCMSLIB00006456969) Stenothricin C M+H has a 1.0 cosine match with Stenothricin C M+H (CCMSLIB00000075068) To pull it out, you can loop through the specs variable until you find it as follows: You can use Stenothricin C, ID CCMSLIB00000075068, as a starting point. Matchms includes built-in functionality to find the similarity between given spectra by looking at the cosine distance between the peak data. Stenothricin spectra form an interesting gene cluster. Now you’re more familiar with matchms and MGF files, let’s walk through using matchms to find clusters of similar spectra. If you don’t have wget, install it first with: As in the previous tutorial, we'll use wget again to view progress as the download is quite large. Use Omigami to find similar spectra, using the Spec2Vec and MS2DeepScore algorithms.Use matchms and Cosine to find similar spectra, with an example spectrum.Install matchms and use it to read the MGF file.Look at the MGF file and see how it compares to JSON.You'll need a computer with at least 8GB of RAM to comfortably load the entire GNPS dataset into memory. You should also be comfortable using pip or conda to install third-party Python packages. To follow along, you should have Python and Jupyter Notebook installed. And you’ll be more familiar with data formats and tools built specifically for metabolomics data analysis. This will get you started with finding clusters of similar spectra. And instead of Pandas, we'll use the specialized Mass Spectrometry libraries matchms (for Cosine distance) and Omigami (for Spec2Vec and MS2DeepScore) to find similar spectra. We’ll demonstrate an alternative method in this article. json file and showed you how to clean and analyze the data using Python and Pandas. In a previous article, GNPS Data with Python and Pandas, we downloaded the GNPS dataset as a.
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Note: while these prices are correct at the time of writing, prices outside the US fluctuate regularly with the exchange rates. Signing up for annual billed monthly locks you into a contract you'll need to pay an exit fee to get out early.Īnnual billed monthly: $20.99 / £19.97 / AU$29.99Īnnual upfront: $239.88 / £238.42 / AU$343.07Īdobe Audition + Creative Cloud All Apps pricesĪnnual billed monthly: $52.99 / £49.94 / AU$76.99Īnnual upfront: $599.88 / £596.33 / AU$871.07 If you're working across mediums like video editing software and photo editors alongside Audition, the plan offers pretty good value for money.Īdobe offers various pricing options for Audition (Image credit: Adobe) How much is Adobe Audition?Īdobe bills for Audition in three ways - monthly, annually billed monthly, and annually paid upfront. The full Creative Cloud subscription will most likely work out cheaper if you’ll use three or more of the included apps. 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Undergraduate Admissions Rhode Island College offers the most affordable high-quality four-year degree in the region. He added that the weather issues were compounded by inexperience on the part of the boaters, who had rented the vessel for an outing that day. The lake, adjacent to Legoland in Winter Haven, was rough on Saturday with 20 mph (32 kph) winds and some whitecaps, Judd said. That child and an 8-year-old girl remained on the boat as it drifted and were rescued safely by the deputies, who had commandeered a fishing boat whose owner cooperated in the effort, Judd said. Feels like: 77 ☏ Few Clouds Wind: 13. December Weather in Winter Haven Florida, United States Daily high temperatures decrease by 4☏, from 76☏ to 72☏, rarely falling below 60☏ or exceeding 83☏. The third person, 38-year-old Velcky Velazques, was rescued by sheriff’s deputies who had been summoned when a 10-year-old girl on the boat dialed 911 on a cellphone. The men became separated from the boat and have not been seen since Saturday evening. Polk County Sheriff Grady Judd said at a news conference that the two men had jumped into Lake Eloise to save a third person from their group, a woman, who was struggling in the water. Winter Haven, FL Weather Conditions Weather Underground star Popular Cities San Francisco, CA warning45 F Clear Manhattan, NY warning44 F Rain Shower Schiller Park, IL (60176) warning35 F. (AP) - Authorities searched Sunday for two boaters who were missing and believed drowned in an accident on a central Florida lake near the Legoland theme park. This progression mode features dozens of engaging and increasingly tricky missions over 3 world maps, with multiple objectives and playstyle options. Brand-New Progression Mode: Experience an exciting new way to play with our mission-based campaign system. Multiple Maps and Themes: Choose from different environments – forest, deserts, tropical islands - and theme options (Western, Sci-fi and more!) to make each park unique. User-Generated Content Tools: For the first time, you will be able to create any item you want in any 3D editing program that works with Unity, and import it into the game for everyone to use! Our robust Steam Workshop integration lets you quickly and easily share your UGC creations with the entire RCTW community! Four themes (Adventure, Sci-fi, Western and Generic) and over 260 premade, tiled pieces give players limitless creative freedom. Piece-by-Piece Builder: Create custom buildings and scenery in-game with our expansive Piece-by-Piece tools. Fully Deformable Terrain with Water: Place amazing rides and change your park’s landscape in full 3D with completely deformable terrain and water. You can make them straight, or for the first time in the franchise, curve them at almost any angle! Curved Paths: Select your unique type of path from a variety of widths, styles, and shapes. Freeform Object Placement: Control and place every single in-game object anywhere on the map at any angle. Expansive Selection of In-Game Objects: Entertain your park guests with tons of different coasters, flatrides, shops, scenery items and more! Innovative 3D Coaster Editor: Easily create the coolest and wildest coasters imaginable with our best track editor ever! Using our spline-based editor, tracks can be fully manipulated in 3D for the first time, allowing you to create any shape you can dream up. If you are looking for a wild ride,then I suggest the Last Riders to you, I would start at the beginning with Razer and work your way to Winter's Touch. The Last Riders is in my top ten favorite series and this story just strengthened my love for these characters, I have been waiting to read this story because I wanted to read Train and Riders as well, so now that they have both been released, I decided to jump back into the little world Jamie Begley created in Treepoint. Begley has a way to draw you into her stories and never let you go. It's an emotional story, I wanted to jump for joy, smash my kindle but I went though a box of tissues. In Winter's touch the author gives us a glimpse of how their relationship is going. How many dicks did Drake make her suck to get voted into that position? Her mouth must be petri dish of infectious diseases! Shit talks any woman that gets so much as a glance from a manĪnd she becomes the towns first fucking lady? Creates a shit storm of trouble for anything with a vaginaģ. She stabs every woman in Treepoint in the backĢ. Last thing: MOTHER FUCKING BLISS IS NOW THE MAYORS WIVE?ġ. How does all that add up to "A beautiful story"? What is it about that situation that brings "tears to peoples eyes"? Is it the mental and emotional abuse that Viper continually dumps on Winter that's causing people to tear up? Because it makes me want to cry for sure. The lack of personal privacy any where in her house, including her own bedroom which has a literal "open door" policy.Īs if all that shit isn't enough, Viper specifically TOLD a club whore to do whatever it took to make Winter hate her, thereby increasing Winter's already overloaded emotional stress exponentially! The ease with which her husband and the club lie to herĩ. The sexual nature of female member initiationĨ. Increased death and destruction caused by club whoresħ. Increased danger via recent attacks on the club, members, and employeesĦ. The sex club lifestyle prohibits her from adopting childrenĥ. Treats her like a 5 year old in front of everyoneīear in mind, Winter is dealing with a shitload of emotional trauma:Ģ. When Winter addresses the bad behavior of club whores herself, Viperģ. Turns it around on her so she feels guilty. Threatens her with a glare or an angry responseĥ. When Winter tries to distance herself a bit or discuss her feelings, Viper:ġ. Changes herself all the time to please Viper and the club whores while they change nothing. Constantly puts up with drama and angst intentionally created by Viper and the club whores.Ħ. Gives up everything to please Viper, the club, and the club whores while they give up nothing.ĥ. Fears her relationship is not secure & Viper will leave her for another woman.Ĥ. Feels worse after spending time with Viper, not better.ģ. Is emotionally drained and feels unfulfilled.Ģ. I hate to say it but it's worse than I feared.ġ. When I saw there was a novella about Winter and Viper I had to read it to see if they were happier than they seemed in the creeptastick "Viper's Run". Claiming the Enemy (Porter Brothers Trilogy) Standing His Ground (Porter Brother's Trilogy)Ģ4. Train's Clash (The Last Riders, Biker Bitches)Ģ0. Keeping What's His (Porter Brothers Trilogy)ġ9. Want updates on new book releases? Sign up for her newsletter at or follow her on Facebook if you want to chat. The entire family shares in their love of nature, music, animals, and outdoor activities and are often found at one of the many beaches or local campgrounds and occasionally, in the hammock with their noses in a book.Jamie Begley is not your average Kentucky girl, and while she does like to cook her southern meals, this down-home momma prefers living in her biker and alien worlds! Tammy is also passionate about family and is the mother of two lovely and active daughters. She understands well, the unique challenges clients may face, while possessing the patience and diligence to see the process through and deliver a property that is just right for her clients. An eight-year tenure in the greater Detroit/Birmingham/Royal Oak area has provided her a unique bi-lateral perspective of the state, as a result, Tammy excels in the art or re-location and second home purchases. Tammy, like Alpine Realty, is committed to quality service and seeing that the entire process- whether buying or selling- is rewarding, timely, and as effortless as possible for all clients. She studied Interior Design and Landscape Architecture at Michigan State University- homes and home ownership have always been at the forefront of her life, interests, and successes.įriendly, knowledgeable, and responsive are but a few of the words clients use when describing Tammy and their interactions with her. A Northern Michigan native, Tammy Hutchinson is passionate about her career in Real Estate. We found the iron perfectly easy to use, but with one caveat: the stock bit is a pencil tip, type “B2” that is fine for the larger surface mount devices but which would in our opinion probably be a little unwieldy for anything smaller than an 0805. To test the iron we assembled a little radio kit, a surface mount design intended for first-time surface mount solderers and thus using fairly substantial 1206 components and SOICs rather than SOPs or smaller integrated circuits. Setting the temperature is a simple case of using the buttons to move the temperature up and down on the OLED display, and once it remains at a particular temperature it stores that setting in its non-volatile memory. It’s quoted as 15 seconds to 300 Celsius at 19 volts in its instruction leaflet, and our iron certainly didn’t disappoint. Heating up, the TS100 may not be as quick as some irons, but it’s no slouch. Our netbook supply had a right-angled plug, and though it wasn’t a nice flexible silicone cable it turned out not to be a significant burden once it was ensured to be out of range of the hot end. The Weller will come fitted with an extra-flexible silicone lead probably designed to work at higher temperatures, by comparison the lead on a cheap power supply is likely to be a stiffer and cheaper affair. However the iron itself is not the whole story, because your choice of power supply and in particular its lead will make a huge difference to how it feels in practice. On its own it is similar in weight and feel to holding a fountain pen, and it is easy to see where comparisons with more expensive irons from the likes of Weller come from. In the hand, the iron is light and easy on the fingers. Maximum power is given as 65 W when supplied with 24 V, while minimum is 17 W with 12 V. We used a 19.5 V netbook supply which was far more than capable of delivering the 40 W the instruction leaflet claims for the iron at 19 V. We hadn’t ordered a power supply with our TS100, but you will doubtless be able to buy one if you don’t have one of the right power level and polarity to hand. The whole assembled unit weighs 30 g, or a shade over an ounce, and has a balance point almost at its centre. The assembled TS100 ironĪssembling the iron is simple enough, the element slots into the receptacle and an Allen screw is tightened to hold it in place. Meanwhile the element unit is about 105 mm (3.15″) long, with an exposed length to the end of the bit of about 70 mm (2.75″). At its rear is a barrel socket for the DC supply alongside a micro-USB socket for firmware and configuration, on its top are a small OLED display and a couple of buttons, and at its front is a receptacle for the element unit. The handle is a plastic wand containing the temperature control electronics about 100 mm (4″) long, and similar in girth to a chunky fountain pen. There was no power supply, you supply your own 12 to 24 V DC to power it. Nestled in foam were the iron handle, a single combined element and bit, and an envelope containing a short instruction leaflet and a click-seal bag with an Allen key and a spare screw to secure the bit. The iron arrived well-packaged in a smart cardboard container that was well up to the task of protecting it through international air mail. We parted with just below £50 (around $60) to place an order for a TS100, and waited for it to arrive so we could see what all the fuss was about. The Miniware TS100 is a relatively inexpensive temperature-controlled soldering iron from China that has made a stealthy entry to the market, and which some online commentators claim to be the equal of far more expensive professional-grade irons. You might believe that this adage represents a cast-iron rule, no iron could possibly combine all three to make a lightweight high-performance tool that won’t break the bank! And until fairly recently you’d have had a point, but perhaps there is now a contender that could achieve that impossible feat. Pick any two of those attributes when you choose an iron, because you’ll never have all three. Temperature-controlled soldering irons can be cheap, lightweight, and good. They need to be able to determine if this is the right place for them. Putting the Power in the Candidates Hands 100% of our Brand Ambassadors complete a video interview. Introducing video interviewing was the biggest game changer. Video interviews provide us the opportunity to assess a candidate’s communication skills and tone-critical skills when searching for customer care team members. In fact, virtual recruiting has the potential to save time and money in an age when recruiters and companies are often inundated with hundreds-or even thousands-of resumes for review.Īs we are a virtual team, we can’t do a face-to-face interview with all candidates. In fact, it’s now easy for a job applicant to not visit the worksite or even shake hands with his or her future boss until right before-or even after-receiving a job offer. Technology has certainly become an integral part of today’s hiring process. Targeting specific cities proves to be more effective. We focus on no more than a dozen markets when looking for new team members rather than posting nationally. We find the outer suburbs of Atlanta and Miami are great labor markets. Because we are virtual, we can go beyond the core area of major metropolitan cities, too. There is a strong labor force with good education and experience in these thriving cities. Looking at markets where there is a lower cost of living and a higher education, including Raleigh and Charlotte, NC and Gainesville and Tallahassee, FL is something we focus on. It’s more than searching in larger cities it’s also looking at smaller metropolitan areas. It’s all about being strategic and going to markets where there is a strong labor force-or more specifically where you have the greatest success of finding qualified individuals. It’s striking that those that take the time to really highlight their qualifications and skills and past successes in an organization really stand out. It is important how candidates present themselves through their resume. In the first three days a position is posted, you will get 80% of the people you ultimately hire. It’s a detailed process to make sure that we are getting the right people for the client and we find over the years that people who respond quickly to the ads are stronger candidates. We kickstarted with the question, in the virtual world, how is your vetting process different?Ĭrucial to vetting and what our team looks for in candidates is the motivation behind candidates and how quickly they respond. We picked her brain on virtual recruiting and getting it right… Maggie is live with us via her virtual home office in Virginia, snug in with her trusty headphones, cold LaCroix, and Stendig calendar (cool factoid about Maggie: it’s the only calendar in the Design Collection of the Museum of Modern Art) If you don’t know, LaCroix is her spirit animal and always close by her side. It’s all about the People – Vetting them, that is She’s so good at it, it’s almost supernatural. Maggie Piper- Presing is our insightful guru that consistently matches the right person to the right position for the right client program. On our website right under her name, the first sentence reads, We sat down with Maggie Piper-Presing, VP of Recruiting at VIPdesk Connect and asked her how she gets virtual recruiting right. On top of that, we do this all virtually. We fill customer service positions all year long, however, we don’t just “fill” them, we hire elite professionals that provide excellent customer service. We know that the nation is full of amazing people that need flexibility with their careers, 25% of the United States is partially or fully remote according to Small Business Trends, that means that by 2020 it could potentially grow to 30% or more.Īt VIPdesk Connect, we have team members located in 37 states providing customer service from their home-based offices. It’s almost 2019 and isn’t nearly every company hiring virtual team members today? Virtual Recruiting and How to do it Well An exclusive interview with Maggie Piper-Presing, VP of Recruiting, VIPdesk Connect        spot removal, mainly burn and Doom Blade The second thing I look at is which kinds of answers are in common use:        protected planeswalkers, in particular Jace, the Mind Sculptor        (accelerated) fatties up to and including Eldrazi        hordes of 1/1s and 2/2s backed by a quasi-combo finish that is usually dependent on the creatures being in play The first one is what threats you’ll often face in the format: Once I know what the decks in the metagame are capable of in general, I like to ask two pivotal questions, so as to get a little more detailed view of what’s going on. Judging by recent tournament results, Ramp (slowly shrinking in popularity with Valakut outnumbering Eldrazi Green), U/B Control, RUG Control, and Weenie aggro (in all three forms) are the most played deck types. These decks actually make me think of (Legacy) Storm combo in that their early game is completely devoted to building up resources, and once a certain threshold is reached, the game is put away in short order.Īt this point, we have a general overview of which strategies are present what we want to know next is how much of the surface they occupy (to step out of the map metaphor: how popular they are). Both try to use a massive amount of acceleration, in particular Rampant Growth-style acceleration, to cast high-end threats early (preferably Primeval Titan). The first strategy to make waves after the rotation was Ramp, in the form of Valakut and Mono-Green Eldrazi. “Here there be giants” – ancient map inscription When I approach a new metagame, what I usually do is class the successful decks according to their underlying strategy to give myself what could be called a map of the metagame. Overall, the new format seems exciting, diverse, and still open to further innovation. You get to run anything from focused beatdown through various combo-ish decks to classic control strategies. Luckily, as the developments after M11 hinted at, those dark days have ended with the rotation of Shards of Alara, and the new Standard format is one I can definitely get behind. Unsurprisingly, the pre-M11 Standard format seemed really bad to me, and the finals of Worlds 2009 and PT San Diego were the most boring PT live casts I’ve ever watched. That being said, when a whole format comes down to doing nothing for two to three turns before starting to drop big creatures on the table, I quickly lose interest. This in turn means that I’ll probably never know the perfect decklist (actually fine-tuning decks takes time), but I’m pretty good at predicting the kind of deck likely to come out on top. I therefore spend a lot more time analyzing the metagame and jotting down rough ideas than actually playing Standard. As I’m likely not going to play in any big Standard tournaments, I’m in the interesting position of not trying to find a deckīut I can instead treat the format like a huge puzzle I’m supposed to solve. I’m obsessed with this game, and I enjoy the mental exercise of trying to figure out what the current Standard format looks like, what I’d do in it. That doesn’t mean I don’t follow the other formats, though. To really enjoy playing Magic, I need my Brainstorms, my Force of Wills and Swords to Plowshares, even the occasional Moat. I love chaining spells, countering stuff, and cheaply killing my opponents’ permanents. My goal wasn’t qualifying for the Pro Tour I wanted to get my hands on the Power Nine to rock Type 1 (Vintage to those of you who don’t share my age). I started playing when Ice Age was new, and I’ve always had a thing for the older cards, even back then. |
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